Section 8.2 - Labeling Oligonucleotides and Nucleic Acids

ثبت نشده
چکیده

Cat # Product Name Unit Size S-7563 SYBR Green I nucleic acid gel stain *10,000X concentrate in DMSO* ............................................................................................................... 500 μL S-7567 SYBR Green I nucleic acid gel stain *10,000X concentrate in DMSO* ............................................................................................................... 1 mL S-7585 SYBR Green I nucleic acid gel stain *10,000X concentrate in DMSO* *special packaging* .............................................................................. 20 x 50 μL S-7564 SYBR Green II RNA gel stain *10,000X concentrate in DMSO* ......................................................................................................................... 500 μL S-7568 SYBR Green II RNA gel stain *10,000X concentrate in DMSO* ......................................................................................................................... 1 mL S-7586 SYBR Green II RNA gel stain *10,000X concentrate in DMSO* *special packaging* ........................................................................................ 20 x 50 μL S-7580 SYBR Green Nucleic Acid Gel Stain Starter Kit ................................................................................................................................................... 1 kit S-21500 SYBR 101, succinimidyl ester ............................................................................................................................................................................ 1 mg S-21501 SYBR 102, succinimidyl ester ............................................................................................................................................................................ 1 mg S-21502 SYBR 103, succinimidyl ester ............................................................................................................................................................................ 1 mg S-11340 SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit *SYTO dyes 17 and 59–64* *50 μL each* ................................................................. 1 kit S-11348 SYTOX Blue nucleic acid stain *5 mM solution in DMSO* ................................................................................................................................. 250 μL S-7020 SYTOX Green nucleic acid stain *5 mM solution in DMSO* .............................................................................................................................. 250 μL S-11368 SYTOX Orange nucleic acid stain *5 mM solution in DMSO* ............................................................................................................................ 250 μL T-3602 TO-PRO-1 iodide (515/531) *1 mM solution in DMSO* .................................................................................................................................... 1 mL T-3605 TO-PRO-3 iodide (642/661) *1 mM solution in DMSO* .................................................................................................................................... 1 mL T-7596 TO-PRO-5 iodide (745/770) *1 mM solution in DMSO* .................................................................................................................................... 1 mL T-3600 TOTO-1 iodide (514/533) *1 mM solution in DMSO* ........................................................................................................................................ 200 μL T-3604 TOTO-3 iodide (642/660) *1 mM solution in DMSO* ........................................................................................................................................ 200 μL Y-3603 YO-PRO-1 iodide (491/509) *1 mM solution in DMSO* .................................................................................................................................... 1 mL Y-3607 YO-PRO-3 iodide (612/631) *1 mM solution in DMSO* .................................................................................................................................... 1 mL Y-3601 YOYO-1 iodide (491/509) *1 mM solution in DMSO* ........................................................................................................................................ 200 μL Y-3606 YOYO-3 iodide (612/631) *1 mM solution in DMSO* ........................................................................................................................................ 200 μL

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Protein-induced changes in DNA structure and dynamics observed with noncovalent site-directed spin labeling and PELDOR

Site-directed spin labeling and pulsed electron-electron double resonance (PELDOR or DEER) have previously been applied successfully to study the structure and dynamics of nucleic acids. Spin labeling nucleic acids at specific sites requires the covalent attachment of spin labels, which involves rather complicated and laborious chemical synthesis. Here, we use a noncovalent label strategy that ...

متن کامل

Internal 32P-labeling of L-deoxyoligonucleotides.

A general two step procedure for the internal labeling of L-deoxyoligonucleotides, Spiegelmers, has been developed. Through radioactive labeling oligonucleotides can easily be detected and monitored in biological samples. T4 polynucleotide kinase is shown to efficiently phosphorylate strands of L-nucleic acids which allows the labeling with phosphorous isotopes such as (32)P. In order to protec...

متن کامل

Tritium labeling of antisense oligonucleotides by exchange with tritiated water.

We describe a simple, efficient, procedure for labeling oligonucleotides to high specific activity (< 1 x 10(8) cpm/mumol) by hydrogen exchange with tritiated water at the C8 positions of purines in the presence of beta-mercaptoethanol, an effective radical scavenger. Approximately 90% of the starting material is recovered as intact, labeled oligonucleotide. The radiolabeled compounds are stabl...

متن کامل

Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations

Site-specific labeling of Escherichia coli ribosomes has allowed application of single-molecule fluorescence spectroscopy and force methods to probe the mechanism of translation. To apply these approaches to eukaryotic translation, eukaryotic ribosomes must be specifically labeled with fluorescent labels and molecular handles. Here, we describe preparation and labeling of the small and large ye...

متن کامل

3' cycle-labeled oligonucleotides with predictable length for primer extension and transgene analysis.

Efficient labeling of short oligos at their 3'-ends was achieved through polymerase chain reaction. The length of cycled-labeled oligos can be accurately predicted by omitting one or more dNTPs in the labeling step. Thus, labeled oligos can be simply column-purified, eliminating the need for tedious gel purification. We demonstrated the effectiveness of this technique in determining the transcr...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2002